The mysterious Espella is being hunted through London by a strange force. With everything she holds dear in danger, she desperately seeks the help of London's most brilliant investigator - Professor Layton! Before the professor and his assistant, Luke Triton, are able to get to work helping Espella, however, she's kidnapped! To make matters even more confusing, while investigating they stumble on an odd book that transports them to the medievel city of Labyrinthia, a place haunted by witches and magic!
Professor Layton isn't the only one on the case, however. Ace attorney Phoenix Wright, along with spirit medium in training Maya Fey, also has an encounter with the book. And, just like the other pair, they're transported to Labyrinthia! Realising that Espella is to be put on trial as a witch, Phoenix decides to do the thing he does best - step in to defend her.
While an investigator and an attorney are the perfect pairing to help someone like Espella, in their respective games they have very different gameplay styles. That's exactly what we see here. Layton must explore the city, piecing together clues, solving puzzles and speaking to the locals. Phoenix Wright, however, has to get to work understanding the strange laws of Labyrinthia so he can cross-examine witnesses - often more than one at once - and prove Espella's innocence!